Jakuwoski

Ítem #: SCP-XXXX

Apple

Apple (Mallus domestica) from a tree affected by SCP-XXXX. On the left are the first signs of the infection, such as lesions in the epicarp (shell) of the fruit, while on the right is a harvested fruit in which there is a progressive replacement of the normal tissue by cellulose and lignin.

Liver%20Pig

Pig liver (Sus scrofa domestica) affected by SCP-XXXX. It is observed that an important portion of the tissue has been replaced by lignified fibers, which would indicate that the specimen reached Phase II of the infection prior to its termination.

Skin

D-3424, 45 days post inoculation of SCP-XXX. The long survival of the subject would indicate that the infection did not spread to the liver and other organs, as usually occurs in Phase II, limiting to the skin and connective tissue. It is possible to observe the presence of cellulose fibers lignified on the skin of the chest and a strong inflammatory reaction in the near tissue not affected.

Object Class: Euclid Keter

Special Containment Procedures: Samples of SCP-XXXX are stored in section of cryopreservation at Biological Research Area-12. Any experimentation with SCP-XXXX should be carried out in a Biosafety level 3 laboratory, and the material used must be incinerated after the procedure has been completed.

Subjects infected with SCP-XXXX must be quarantined and treated, if the infection is in Phase I, with a combination of 250 mg of chloramphenicol and 500 mg of streptomycin every 8 hours for a period of 30 days, in addition with necessary surgical procedures for remove altered tissues, if the infection reached the Phase II. If the subject does not respond to antibiotic treatment, it should be terminated and incinerated. If the individual die as consequence of the infection, its remains and any material with which has been in contact must be incinerated, in order to avoid the possibility of additional contagion.

Plants infected with SCP- XXXX must be cut and incinerated at temperature higher than 800 °C. Due to discovery that SCP-XXXX can survive in the soil as saprophyte, the finding area should be treated with 10% copper sulphate solution and kept uncultivated for a period of 1 year, after which sampling will be carried out to confirm the eradication of the pathogen.
Animals infected with SCP-XXXX must be terminated and their remains incinerated, as part of the measures to prevent propagation.

Currently the Foundation has a test to detect the infection of SCP-XXXX in Phase I and is working in the development of a vaccine.

Description: SCP-XXXX is a organism called temporally as Dendrobacteria sp. It corresponds to a pathogenic bacterium, whose infection causes the formation of cellulose and lignin fibers than invade the host tissues, causing from local injures to death. It has been determined that this organism has 81.33% homology in its genome with Pseudomonas Syringae and 8.4% with Agrobacterium tumefaciens. Unlike the different strains of P. Syringae1, SCP-XXXX has a wide range of organisms that can infect, including plant species of the genera Triticum, Hordeum, Pisum, Malus, Oleae, Acer, Prunus, Citrus, Cocoas and animal species of the genera Mus, Gallus, Sus, Bos and Homo.

Additionally, it has been observed that SCP-XXXX could lack the tumorigenic capacity observed in P. syringae and A. tumefaciens, showing instead the ability to produce cellulose fibers from glucose and fructose available in the medium, which are secreted by the microorganism on its cellular surface and used to adhere to the surface of the host cell. Subsequently, using a mechanism similar to that used by A. tumefaciens, SCP-XXXX injects proteins and genetic material into the host cell, inducing a series of metabolic changes that lead to the infected cell producing, from glucose and starch, cellulose and phenolic compounds, resulting in the alteration of infected tissues and the formation of structures similar to wood in parts of the plant usually do not lignified, such as leaves and fruits.

In animals, including humans, the infection for SCP-XXXX is usually is contracts through consumption of infected fruits with the pathogen2, than are describes as "fibrous" or "with splinters embedded in the pulp” for those who have ingested them. The microorganism would survive the conditions of the digestive tract thanks to a cover of polysaccharides that possesses, after which it adheres to the intestinal epithelium, infecting and using it to enter the bloodstream. This stage of the infection is called Phase I, happens 5-14 days post inoculation and in it occurs the death of 73% of the individuals affected by the pathogen, as a consequence of pulmonary thrombosis and myocardial infarction, due to the formation of clots around the cellulose fibers secreted by the microorganism and subsequent obstruction of main blood vessels.

In individuals who survive Phase I, the infection progresses to Phase II, in which there is a transfection3 by SCP-ES-XXX of fibroblasts of different areas of the body, which begin to secrete cellulose and lignin instead of Glycosaminoglycans4 occurring a displacement of the proteins of the matrix by these polymers, and causing a severe inflammatory reaction and necrosis in the affected area, be necessary, in most of the cases, remove the lignified tissue, to save the life of the patient. When the infection is in the liver and muscles, happens a displacement of the glycogen for cellulose and lignin, which leads to the death of 95% of the affected. This phase occur 14-23 days post inoculation.

In the few subjects that can survive Phase II, it produce the Phase III, 25-45 days post inoculation, in which the infection is propagated to Skeletal and smooth musculature, where the tissue is progressively replaced with lignified fibers, occurring the death in the 100% of the cases.

There have been cases in which SCP-XXXX achieves, post death of the patient, surpass the normal microbial flora and continue the process of colonization, causing the conversion of up to 76% of the connective and muscular tissues of the victim in fibers of cellulose and lignin.

For more information please review the following publications:

Wood, S. Elliot, C. Description of the novel plant pathogenic agent Dendrobacteria. Journal of Emergent Plants Diseases 5, 27-37 (2012)

Mann, E. Stone, R. Characterization of the mechanisms of infection of Dendrobacteria. Infections Factors 6 33-41 (2015)

Ryo, M. Wall, N. Inter specific infectious ability in Dendrobacteria. How it is possible? Interspecific Pathogens 3 45-53(2016)